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primary hippocampal neuron cell culture  (Dawley Inc)

 
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    Dawley Inc primary hippocampal neuron cell culture
    Primary Hippocampal Neuron Cell Culture, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+cultured+hippocampal+neurons/cells+muscle+primary+smooth+vascular/pmc13061559-2-5-11
    Average 86 stars, based on 1 article reviews
    primary hippocampal neuron cell culture - by Bioz Stars, 2026-09
    86/100 stars

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    Cell Culture:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Patch Clamp:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Microscopy:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Immunostaining:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Sandwich ELISA:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Sonication:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Centrifugation:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    DC Protein Assay:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Staining:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Confocal Microscopy:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Nucleic Acid Electrophoresis:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Western Blot:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Phospho-proteomics:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Activity Assay:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    TUNEL Assay:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Expressing:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Transgenic Assay:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Injection:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Saline:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Isolation:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Incubation:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Transfection:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Imaging:

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: Western blotting analysis The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling
    Article Snippet: The data in , and were done in intact neurons or isolated nuclei of primary cultured hippocampal neurons from postnatal day 1 Sprague-Dawley rat.

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.

    Article Title: Mechanisms of action of phytochemicals from medicinal herbs in the treatment of Alzheimer's disease.
    Article Snippet: First author, year [ref] Study design Treatments Results Zou et al., 2013 [41] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓ ROS levels ↑ phosphorylation of GSK3β at Ser9 Lin et al., 2012 [42] Primary cultured hippocampal neurons from Sprague –Dawley rats Pretreatment with 3, 10, 30, 100 μM puerarin for 2 hours and addition with 20 μM Aβ25–35 for 24 h ↑ cell viability ↓GSK3β activity ↑ phosphorylation of Akt ↑ level of GSH‐Px ↑ level of CAT activity Zhang et al., 2008 [43] PC12 cells (rat pheochromocytoma) Preincubation with 0.1, 1, and 10 μM puerarin for 30min and addition with 50 μMAβ25–35 for 24 h ↑ cell viability ↓ TUNEL-positive cells ↓ annexin V+/PI− fraction ↑ expression of Bcl-2 ↓ expression of Bax ↓ caspase-3 Zhang et al., 2011 [44] mitochondrial transgenic neuronal cell used Rho° SH-SY5Y cells lacking mtDNA Treatment with 0.1, 1, and 10 μM puerarin for 24 hours ↑ cell viability of SAD cybrids ↓ annexin V+/PI− fraction ↓ intracellular ROS generation ↑ expression of Bcl-2 ↓ expression of Bax ↓ phosphorylation of JNK and p38 ↓ caspase-3 activity Li et al., 2010 [45] Male Sprague–Dawley rats Intracerebroventricular injection of 10 μg/μL Aβ1–42 and after 3 days, orally administration bygavagewith puerarin for 28 days; Six experimental groups: (1) sham-operated group (2) saline-treated Aβ1–42 group (3) donepezil-treated Aβ1–42 group (4) 25mg/kg puerarin-treated Aβ1–42 group (5) 50mg/kg puerarin-treated Aβ1–42 group (6) 25mg/kg puerarin-treated Aβ1–42 group ↓MWM task ↑ number of crossings over a platform position ↓ TUNEL-positive cells ↑ phosphorylation of Akt-ser473 and ‐Bad levels ↔ unchange on total Akt and Bad levels ↓ caspase-9 protein andmRNA levels ↑ Increase; ↓ decrease;↔ no difference 1254 Kim MH et al. Mechanisms of Action... Planta Med 2014; 80: 1249–1258 Reviews T hi s do cu m en t w as d ow nl oa de d fo r pe rs on al u se o nl y. U na ut ho riz ed d is tr ib ut io n is s tr ic tly p ro hi bi te d. 1255 Reviews puerarin attenuated apoptosis by regulating anti-apoptotic and pro-apoptotic molecules.

    Article Title: In Vitro Neuroprotection of Rat Hippocampal Neurons by Manninotriose and Astragaloside IV Against Corticosterone-Induced Toxicity
    Article Snippet: Primary cultured hippocampal neurons from newborn Sprague Dawley rats were treated with CORT in the absence or presence of Manninotriose and AS-IV.

    Article Title: SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.
    Article Snippet: Primary hippocampal neuron culture Primary cultured hippocampal neurons were prepared from Sprague-Dawley rats at PND 0.

    Article Title: Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons
    Article Snippet: The proteins extracted from the hippocampal tissues of Sprague-Dawley rats and primary cultured hippocampal neurons were denatured at 95 °C for 5 min and were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Antiretroviral drugs promote amyloidogenesis by de-acidifying endolysosomes
    Article Snippet: Primary cultures of rat hippocampal neurons As previously described ( Buscemi et al., 2007 ; Hui et al., 2012b ), primary cultured hippocampal neurons were prepared from Sprague-Dawley rats.



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    Effects of 12-mer-19 (19) and 12-mer-NAc (NAc) on the function excitatory synapses (A-C) and inhibitory synapses (D-E). A) Traces of mEPSC recordings from primary rat hippocampal neurons. B) Treatment of 12-mer-19 resulted in a reduction in mEPSC frequency. There was no significant change between control (Con) and 12-mer-NAc-treated groups. C) There was a significant decrease in EPSC amplitude between 12-mer-19 and control groups but not between 12-mer-19 and 12-mer-NAc groups. D) Traces of mIPSC recordings from primary rat hippocampal neurons. E and F) No significant difference was observed among all groups in the frequency (E) and amplitude (F) of mIPSC. ****p<0.0001 or *p=0.01 by one-way ANOVA with posthoc Bonferroni’s multiple comparison tests. N=21-24 cells from 6 independent experiments for mEPSC. N=19-22 cells from 3 independent experiments for mIPSC. Error bars represent SEM.

    Journal: Glycobiology

    Article Title: A synthetic HS structure selectively impairs the morphology and function of excitatory synapse by disrupting neurexin1 interactions

    doi: 10.1093/glycob/cwaf039

    Figure Lengend Snippet: Effects of 12-mer-19 (19) and 12-mer-NAc (NAc) on the function excitatory synapses (A-C) and inhibitory synapses (D-E). A) Traces of mEPSC recordings from primary rat hippocampal neurons. B) Treatment of 12-mer-19 resulted in a reduction in mEPSC frequency. There was no significant change between control (Con) and 12-mer-NAc-treated groups. C) There was a significant decrease in EPSC amplitude between 12-mer-19 and control groups but not between 12-mer-19 and 12-mer-NAc groups. D) Traces of mIPSC recordings from primary rat hippocampal neurons. E and F) No significant difference was observed among all groups in the frequency (E) and amplitude (F) of mIPSC. ****p<0.0001 or *p=0.01 by one-way ANOVA with posthoc Bonferroni’s multiple comparison tests. N=21-24 cells from 6 independent experiments for mEPSC. N=19-22 cells from 3 independent experiments for mIPSC. Error bars represent SEM.

    Article Snippet: Primary rat hippocampal neuron cultures were prepared from embryonic day 18 rat (Charles River, CD 001) using the Banker-style culture method ( Kaech and Banker 2006 ).

    Techniques: Control, Comparison